Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of dendritic cell subsets in human tonsils and blood

ABSTRACT: An experiment was performed to further analyze the functional characteristics and relationship of the DC subsets in human tonsils and blood.

ORGANISM(S): Homo sapiens

SUBMITTER: Malin Lindstedt

PROVIDER: E-TABM-34 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Gene family clustering identifies functionally associated subsets of human in vivo blood and tonsillar dendritic cells.

Lindstedt Malin M Lundberg Kristina K Borrebaeck Carl A K CA

Journal of immunology (Baltimore, Md. : 1950) 20051001 8

Human dendritic cells (DCs) are a distinct but heterogeneous lineage of APCs operating as the link between innate and adaptive immune responses, with the function to either maintain tolerance or trigger immunity. The DC lineage consists of several subpopulations with unique phenotypes; however, their functional characteristics and transcriptional similarities remain largely unknown. To further characterize the phenotypes and transcriptomes of the subsets, we purified myeloid CD16(+), blood DC Ag ...[more]

PMID: 16210585

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Project description:Dendritic cells (DCs) are a complex group of cells which play a critical role in vertebrate immunity. Spleen or lymph node resident DCs are subdivided into conventional DC (cDC) subsets (CD11b and CD8alpha in mouse; BDCA1 and BDCA3 in man) and plasmacytoid DCs (pDCs). It is currently unclear if these various DC populations belong to a unique hematopoietic lineage and if the subsets identified in the mouse and human systems are evolutionary homologs. To bring novel insights into these questions, we sought conserved genetic signatures for these DCs through the analysis of a compendium of genome-wide expression profiles of mouse or human leukocytes. We show through clustering analysis that all spleen resident DC subsets form a distinct branch within the leukocyte family tree, and reveal a transcriptomal signature evolutionary conserved in all these DC subsets. Moreover we identify a large gene expression program shared between mouse and human plasmacytoid DCs, and smaller conserved profiles shared between mouse and human cDC subsets. Finally, we use compendium analysis to re-evaluate the classification of interferon-producing killer DCs (IKDCs) and lin-CD16+HLA-DR+ cells, which have both been claimed to be DCs, and show that these cells are more closely linked to NK or myeloid cells, respectively. Our study thus provides a unique resource for future investigation of the evolutionarily conserved molecular pathways governing the ontogeny and functions of leukocyte subsets, especially DCs. Experiment Overall Design: This study includes data from cell sort purified dendritic cells, B cells, NK cells, and CD8 T cells. 2 or 3 independent replicates were made for each cell type. The genearray was performed in an attempt to investigate the relationships between DCs subsets and with other leukocytes, in mouse, in human, and between these 2 species. To this end, public data for mouse CD4 T cells and macrophages, as well as public data for human leukocyte subsets were also used in the analysis. The results have led to the identification of specific transcriptional programs conserved between human and mouse dendritic cell subsets.

Dataset Information

Transcription profiling of dendritic cell subsets in human tonsils and blood

Publications

Gene family clustering identifies functionally associated subsets of human in vivo blood and tonsillar dendritic cells.

Similar Datasets

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