Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of M and S molecular forms of Anopheles gambiae at each of three developmental stages


ABSTRACT: We examined patterns of gene expression in two independent colonies of both M and S molecular forms of Anopheles gambiae at each of three developmental stages of interest: late larvae, sugar-fed virgin females, and gravid females. For each colony, replicates were derived from independent RNA samples extracted from different cohorts to ensure that trends were reproducible. In addition, each replicate was derived from larvae (adults) drawn from three pans (cages) to minimize the contribution of any individual pan to variation between samples. Data were obtained from a total of five biological replicates per mosquito colony.

ORGANISM(S): Anopheles gambiae

SUBMITTER: Karine Mouline 

PROVIDER: E-TABM-344 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Differential gene expression in incipient species of Anopheles gambiae.

Cassone Bryan J BJ   Mouline Karine K   Hahn Matthew W MW   White Bradley J BJ   Pombi Marco M   Simard Frederic F   Costantini Carlo C   Besansky Nora J NJ  

Molecular ecology 20080421 10


A speciation process is ongoing in the primary vector of malaria in Africa, Anopheles gambiae. Assortatively mating incipient species known as the M and S forms differentially exploit larval breeding sites associated with different ecological settings. However, some ongoing gene flow between M and S limits significant genomic differentiation mainly to small centromere-proximal regions on chromosomes X and 2L, termed 'speciation islands' with the expectation that they contain the genes responsibl  ...[more]

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