Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Chromatin immunopreciptiation of human lymphoblastoid cells to identify histone H3K9/14 acetylation regions at common fragile sites


ABSTRACT: Map the histone H3K9/14 acetylation regions of in human cells at 7 common fragile sites and their flanking non-fragile sequences as well as a 200kb containing the rare fragile site FRAXA, a 1,075kb non-fragile region on chr22, a hyperacetylated region HALPHA44, and a heterochromatic region HET405. The acetylated regions were mapped in untreated, aphidicolin(APH)-treated, trichostatin(TSA)-treated, and TSA plus APH-treated cells by combining the chromatin-immunoprecipitation with a tiled microarray platform (ChIP-chip).

ORGANISM(S): Homo sapiens

SUBMITTER: Isabelle Lucas 

PROVIDER: E-TABM-347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Common fragile sites are characterized by histone hypoacetylation.

Jiang Yanwen Y   Lucas Isabelle I   Young David J DJ   Davis Elizabeth M EM   Karrison Theodore T   Rest Joshua S JS   Le Beau Michelle M MM  

Human molecular genetics 20090828 23


Common fragile sites (CFSs) represent large, highly unstable regions of the human genome. CFS sequences are sensitive to perturbation of replication; however, the molecular basis for the instability at CFSs is poorly understood. We hypothesized that a unique epigenetic pattern may underlie the unusual sensitivity of CFSs to replication interference. To examine this hypothesis, we analyzed chromatin modification patterns within the six human CFSs with the highest levels of breakage, and their sur  ...[more]

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