Chromatin immunoprecipitation of liver tissue from humans, wild type mice and a Down syndrome mouse model containing human chromosome 21 to determine where genetic sequence or nuclear environment directs tissue specific-specific transcription factor binding

ABSTRACT: Homologous sets of transcription factors direct conserved tissue-specific transcription, yet transcription factor binding events diverge rapidly between closely related species. We used hepatocytes from a Down syndrome mouse model containing human chromosome 21 to determine whether human genetic sequence or mouse nuclear environment primarily determines tissue-specific transcriptional regulation. Virtually all transcription factor binding locations, transcription initiation events and the resulting gene expression observed in human hepatocytes are recapitulated across the entire human chromosome 21 in the mouse nucleus. Thus, in homologous tissues, genetic sequence is largely responsible for directing transcriptional programs, and interspecies differences in epigenetics, cellular environment, and transcription factors themselves play secondary roles.

ORGANISM(S): Homo sapiens

SUBMITTER: Nuno Barbosa-Morais 

PROVIDER: E-TABM-474 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress