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Chromatin immunoprecipitation analysis of human hepatocyte regulatory circuitry

ABSTRACT: We mapped the transcriptional regulatory circuitry for six master regulators in human hepatocytes using chromatin immunoprecipitation and high-resolution promoter microarrays. The results show that these regulators form a highly interconnected core circuitry, and reveal the local regulatory network motifs created by regulator-gene interactions. Auto-regulation was a prominent theme among these regulators. We found that hepatocyte master regulators tend to bind promoter regions combinatorially and that the number of transcription factors bound to a promoter corresponds with observed gene expression. Our studies reveal portions of the core circuitry of human hepatocytes.

ORGANISM(S): Homo sapiens

SUBMITTER: Elizabeth Herbolsheimer

PROVIDER: E-WMIT-9 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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