Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Dominic Schmidt
PROVIDER: E-TABM-485 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Schmidt Dominic D Stark Rory R Wilson Michael D MD Brown Gordon D GD Odom Duncan T DT
PloS one 20081112 11
Chromatin immunoprecipitation followed by high-throughput (HTP) sequencing (ChIP-seq) is a powerful tool to establish protein-DNA interactions genome-wide. The primary limitation of its broad application at present is the often-limited access to sequencers. Here we report a protocol, Mab-seq, that generates genome-scale quality evaluations for nucleic acid libraries intended for deep-sequencing. We show how commercially available genomic microarrays can be used to maximize the efficiency of libr ...[more]