Unknown,Transcriptomics,Genomics,Proteomics

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Identification of Sox3 targets in mouse neural progenitor cells

ABSTRACT: Sox3 has been shown to be expressed within neural progenitors of the developing mouse central nervous system. However, identification of Sox3 targets within neural progenitors has remained elusive. Using microarrays we compared populations of in vitro derived mouse neural progenitor cells, with or without Sox3, to identify putative Sox3 neural progenitor targets. Mouse R1 ES cells, with or without Sox3, were differentiated into neural progenitor cells using the standard N2B27 protocols. At the fourth day of N2B27 differentiation, over two independent series, RNA was extracted from both Sox3 positive and Sox3 null populations and hybridization on a GeneChip Mouse Gene 1.0 ST Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Nicholas Rogers 

PROVIDER: E-GEOD-53760 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Dbx1 is a direct target of SOX3 in the spinal cord.

Rogers Nicholas N   McAninch Dale D   Thomas Paul P  

PloS one 20140421 4

SoxB1 sub-family of transcriptional regulators are expressed in progenitor (NP) cells throughout the neuroaxis and are generally downregulated during neuronal differentiation. Gain- and loss-of-function studies indicate that Sox1, Sox2 and Sox3 are key regulators of NP differentiation and that their roles in CNS development are largely redundant. Nevertheless, mutation of each SoxB1 individually results in a different array of CNS defects, raising the possibility that SoxB1 proteins have subtly  ...[more]

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