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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Methylation profiling of mouse liver, kidney, spleen and cerebrum using D-REAM

ABSTRACT: DNA methylation profiling by D-REAM.

Additional data files (.BED files and heatmap file) are available in the FTP directory for this experiment FTP directory

ORGANISM(S): Mus musculus

SUBMITTER: Shinya Sato

PROVIDER: E-TABM-551 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) in mouse promoter regions demonstrating tissue-specific gene expression.

Yagi Shintaro S Hirabayashi Keiji K Sato Shinya S Li Wei W Takahashi Yoko Y Hirakawa Tsutomu T Wu Guoying G Hattori Naoko N Hattori Naka N Ohgane Jun J Tanaka Satoshi S Liu X Shirley XS Shiota Kunio K

Genome research 20081029 12

DNA methylation constitutes an important epigenetic regulation mechanism in many eukaryotes, although the extent of DNA methylation in the regulation of gene expression in the mammalian genome is poorly understood. We developed D-REAM, a genome-wide DNA methylation analysis method for tissue-dependent and differentially methylated region (T-DMR) profiling with restriction tag-mediated amplification in mouse tissues and cells. Using a mouse promoter tiling array covering a region from -6 to 2.5 k ...[more]

PMID: 18971312

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