Translation profiling of fission yeast wild type and rng3-65 fission yeast to identify changes in th enumber of ribosomes associated with specific transcripts

ABSTRACT: Genome-wide translational profiling of rng3-65 compared to wild type cells. We used sucrose gradients to separate RNAs according to the number of associated ribosomes (a surrogate for translational efficiency). Preparation of the extracts and fractionation was carried out as described in Lackner et al, 2007 (Mol Cell 26(1):145-55). The fractions were pooled into four groups (1 closest to the top, i.e. not associated with ribosomes and 4 closest to the bottom, i.e., associated with polysomes). RNA was extracted from the pools and the corresponding pools from wild type and mutant cells were directly compared using DNA microarrays. Changes in translation are expected to alter the number of ribosomes associated with specific transcripts, and therefore result in a redistribution of the RNAs across the different fractions.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Juan Mata 

PROVIDER: E-TABM-565 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress