Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

24 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Microarray-based comparison of RSC, Condensin and Cohesin

ABSTRACT: Total mRNA was isolated from parallel cultures of two Matalpha clones of each of the 7 temperature sensitive strains: STH1ts, SMC2ts, SMC3ts, STH1ts SMC2ts, STH1ts SMC3ts, SMC2ts SMC3ts, and STH1ts SMC2ts SMC3ts. The two biological replicates were processed in parallel and one was conjugated to Cy3, the other one to Cy5 dye. A competitive Cy3-Cy5 hybridization was then performed against aliquots of a pool of mRNA obtained from several clones of the congenic wild type strain. This experimental strategy was chosen to permit horizontal comparisons across the seven mutant strains.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Anne-Marie Collin

PROVIDER: E-UMCU-21 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of matching human primary head and neck squamous cell carcinomas and cervical lymph node metastases.

Project description:An experiment was performed to investigate the perservation of gene expression upon metastasis of primary head and neck squamous cell carcinomas to the cervical lymph node.

2006-10-23 | E-TABM-114 | biostudies-arrayexpress

Transcription profiling of human primary B-cell vs monocyte-derived dendritic cells

Project description:Genome-wide RNAi screen reveals new pathways for MHC class II antigen presentation

2010-12-18 | E-MTAB-192 | biostudies-arrayexpress

Transcription profiling of heptatocytes from mice given a high-copper diet

Project description:In order to study the physiological consequences of a high-copper diet on hepatic gene expression, 6 mM CuCl2 was added to the drinking water for a period of 1 month. After this period, livers of seven control mice and eight copper-treated mice were isolated and were subjected to microarray analysis and copper measurements. The hepatic gene expression profile of copper-treated mice was compared to non-treated mice using a pooled reference.

2007-06-01 | E-MEXP-745 | biostudies-arrayexpress

Transcription profiling of human hepatocytes in response to copper overload at nine time points

Project description:Nine time points for microarray analysis were chosen to study early and late transcriptional responses in copper metabolism upon copper overload in HepG2 cells. Samples of copper-treated cells were hybridized using non-treated samples as a reference.

2007-06-01 | E-MEXP-753 | biostudies-arrayexpress

Chromatin immunoprecipitation genome wide map of nucleosome acetylation and methylation in Yeast

Project description:Genomes are packaged into nucleosomes whose position and modification state can profoundly influence regulation of gene expression. We have established new ChIP-based high-resolution genome-wide maps of histone acetylation and methylation that take into account changes in nucleosome occupancy at actively transcribed genes. These maps provide a more accurate picture of nucleosome occupancy and the nature of modifications associated with transcriptional activity in this eukaryotic genome. We describe the combination of modifications that occur at the average transcribed gene.

2005-08-25 | E-WMIT-3 | biostudies-arrayexpress

Chromatin immunoprecipitation genome wide occupancy of 4 different chromatin regulating complexes encoded in yeast

Project description:We explore the genome-wide occupancy of 4 different chromatin regulating complexes encoded in S. cerevisiae. We provide the data for histone acetyltransferases Gcn5 and Esa1 and histone deacetylases Hst1 and Rpd3/Sin3 under rich growth condition (YPD medium). We also include the occupancy data for RNA polymerase II under the same growth condition.

2005-11-03 | E-WMIT-2 | biostudies-arrayexpress

Chromatin immunoprecipitation genome wide binding site identification in yeast

Project description:Genome Wide Location: binding site identification

2013-01-30 | E-WMIT-1 | biostudies-arrayexpress

Transcription profiling of six pairs of affected and non-affected male F2 mice selected on the basis of their motor activity levels

Project description:Mice were obtained from in house breeding of C57BL/6J and C57BL/6J-Chr 1A/Na breeding pairs (Jackson Laboratories, USA). To produce F1 hybrids, C57BL/6J females were mated with C57BL/6J-Chr 1A/Na males. The F1 hybrids were intercrossed, producing 82 F2 progeny (41 males and 41 females). Microarray analysis was performed on six pairs of affected and non-affected male animals from the F2 progeny selected on the basis of their motor activity levels (average daily levels of distance moved over a 3 days recording: 768±74 cm/hr (affected) versus 1765±175 cm/hr (non-affected)(p<0.0001).

2006-12-31 | E-UMCU-22 | biostudies-arrayexpress

Transcription profiling of homozygous Commd1 null mouse embryos to elucidate the underlying mechanism leading to embryonic lethality

Project description:A gene expression study using microarray analysis was performed to elucidate the underlying mechanism leading to embryonic lethality in homozygous Commd1 null (Commd1-/-) mouse embryos. A gene expression profile of 9.5 dpc Commd1-/- embryos were generated and were compared to a gene expression profile of both 8.5 dpc and 9.5 dpc normal embryos.

2006-09-01 | E-MEXP-832 | biostudies-arrayexpress

Transcription profiling yeast using different amounts if spiked in external controls to assess statistical methods for microarray data analysis based on spike

Project description:Evaluation of statistical methods for microarray data analysis using spiked-in external controls.

2006-04-03 | E-UMCU-19 | biostudies-arrayexpress