Dataset Information

Quantification of bowel ischaemia using real-time multispectral Single Snapshot Imaging of Optical Properties (SSOP).

ABSTRACT:

Background

Single snapshot imaging of optical properties (SSOP) is a relatively new non-invasive, real-time, contrast-free optical imaging technology, which allows for the real-time quantitative assessment of physiological properties, including tissue oxygenation (StO2). This study evaluates the accuracy of multispectral SSOP in quantifying bowel ischaemia in a preclinical experimental model.

Methods

In six pigs, an ischaemic bowel segment was created by dividing the arcade branches. Five regions of interest (ROIs) were identified on the bowel loop, as follows: ROI 1: central ischaemic; ROI 2: left marginal; ROI 3: left vascularised; ROI 4: right marginal; and ROI 5: right vascularised. The Trident imaging system, specifically developed for real-time tissue oxygenation imaging using SSOP, was used to image before (T0) and after ischaemia induction. Capillary and systemic lactates were measured at each time point (T0, T15, T30, T45, T60), as well as StO2 values acquired by means of SSOP (SSOP-StO2).

Results

The mean value of SSOP-StO2 in ROI 1 was 30.08 ± 6.963 and was significantly lower when compared to marginal ROIs (ROI 2 + ROI 4: 45.67 ± 10.02 p = < 0.0001), and to vascularised ROIs (ROI 3 + ROI 5: 48.08 ± 7.083 p = < 0.0001). SSOP-StO2 was significantly correlated with normalised lactates r = - 0.5892 p < 0.0001 and with histology r =- 0.6251 p = 0.0002.

Conclusion

Multispectral SSOP allows for a contrast-free accurate assessment of small bowel perfusion identifying physiological tissue oxygenation as confirmed with perfusion biomarkers.

SUBMITTER: Rodriguez-Luna MR

PROVIDER: S-EPMC10017661 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Publications

Quantification of bowel ischaemia using real-time multispectral Single Snapshot Imaging of Optical Properties (SSOP).

Rodríguez-Luna María Rita MR Okamoto Nariaki N Cinelli Lorenzo L Baratelli Luca L Ségaud Silvère S Rodríguez-Gómez Adriana A Keller Deborah S DS Zonoobi Elham E Bannone Elisa E Marescaux Jacques J Diana Michele M Gioux Sylvain S

Surgical endoscopy 20221128 3

<h4>Background</h4>Single snapshot imaging of optical properties (SSOP) is a relatively new non-invasive, real-time, contrast-free optical imaging technology, which allows for the real-time quantitative assessment of physiological properties, including tissue oxygenation (StO2). This study evaluates the accuracy of multispectral SSOP in quantifying bowel ischaemia in a preclinical experimental model.<h4>Methods</h4>In six pigs, an ischaemic bowel segment was created by dividing the arcade branch ...[more]

PMID: 36443562

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Project description:BackgroundIdentifying liver ischemia is crucial in liver surgery. This study aimed to develop a hemi-hepatic ischemia model for assessing liver ischemia using single snapshot imaging of optical properties (SSOP), a noninvasive optical imaging modality that provides real-time measurements of tissue oxygen saturation (StO2).Materials and methodsTwelve swine were randomly assigned to two groups: One undergoing total vascular inflow occlusion (TVIO) and the other undergoing hepatic artery occlusion (HAO). Preoperative 3D CT scans were used to locate the left-sided hepatic arteries and portal veins, which were clamped during surgery. Real-time SSOP imaging was conducted to measure StO2 in three lobes-the left lateral lobe (LL), left medial lobe (LM), and right medial lobe (RM)-as well as capillary lactate levels and Doppler blood flow. Measurements were recorded at baseline (T0), during ischemia (T1, 30 min after clamping), and during reperfusion (T2, 30 min after declamping).ResultsIn the TVIO group, SSOP imaging revealed a distinct demarcation line on the liver surface. StO2 levels measured by SSOP significantly decreased from T0 to T1, dropping by 29.8% in the LL (46.0 ± 5.1 vs. 16.2 ± 5.1%, p = 0.011) and 36.3% in the LM (42.7 ± 5.9 vs. 6.4 ± 4.0%, p = 0.001). Additionally, capillary lactate levels increased substantially in the LL (1.3 ± 0.4 vs. 8.5 ± 2.4 mmol/L, p = 0.041) and in the LM (1.3 ± 0.4 vs. 8.2 ± 2.1 mmol/L, p = 0.021). In contrast, the HAO group showed a less pronounced reduction in StO2: 13.6% in the LL (32.7 ± 6.4 vs. 19.1 ± 5.4%, p = 0.007) and 19.8% in the LM (35.3 ± 8.2 vs. 15.5 ± 5.8%, p = 0.011), with no significant increase in capillary lactate levels. An inverse correlation was found between StO2 and capillary lactate levels (r = - 0.76, p < 0.001).ConclusionSSOP is a real-time, contrast-free imaging technique that effectively evaluates liver ischemia by accurately measuring tissue oxygenation, as validated by perfusion biomarkers.

Dataset Information

Quantification of bowel ischaemia using real-time multispectral Single Snapshot Imaging of Optical Properties (SSOP).

Background

Methods

Results

Conclusion

Publications

Quantification of bowel ischaemia using real-time multispectral Single Snapshot Imaging of Optical Properties (SSOP).

Similar Datasets

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