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Real-Time Volumetric Phase Monitoring: Advancing Chemical Analysis by Countercurrent Separation.

ABSTRACT: Countercurrent separation (CCS) utilizes the differential partitioning behavior of analytes between two immiscible liquid phases. We introduce the first platform ("CherryOne") capable of real-time monitoring, metering, and control of the dynamic liquid-liquid CCS process. Automated phase monitoring and volumetrics are made possible with an array of sensors, including the new permittivity-based phase metering apparatus (PMA). Volumetric data for each liquid phase are converted into a dynamic real-time display of stationary phase retention (Sf) and eluent partition coefficients (K), which represent critical parameters of CCS reproducibility. When coupled with the elution-extrusion operational mode (EECCC), automated Sf and K determination empowers untargeted and targeted applications ranging from metabolomic analysis to preparative purifications.

SUBMITTER: Pauli GF

PROVIDER: S-EPMC4509944 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Real-Time Volumetric Phase Monitoring: Advancing Chemical Analysis by Countercurrent Separation.

Pauli Guido F GF Pro Samuel M SM Chadwick Lucas R LR Burdick Thomas T Pro Luke L Friedl Warren W Novak Nick N Maltby John J Qiu Feng F Friesen J Brent JB

Analytical chemistry 20150708 14

Countercurrent separation (CCS) utilizes the differential partitioning behavior of analytes between two immiscible liquid phases. We introduce the first platform ("CherryOne") capable of real-time monitoring, metering, and control of the dynamic liquid-liquid CCS process. Automated phase monitoring and volumetrics are made possible with an array of sensors, including the new permittivity-based phase metering apparatus (PMA). Volumetric data for each liquid phase are converted into a dynamic real ...[more]

PMID: 26152934

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