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Mechanoenzymatic reactions for the hydrolysis of PET.

ABSTRACT: Recent advances in the enzymatic degradation of poly(ethylene terphthalate) (PET) have led to a number of PET hydrolytic enzymes and mutants being developed. With the amount of PET building up in the natural world, there is a pressing need to develop scalable methods of breaking down the polymer into its monomers for recycling or other uses. Mechanoenzymatic reactions have gained traction recently as a green and efficient alternative to traditional biocatalytic reactions. For the first time we report increased yields of PET degradation by whole cell PETase enzymes by up to 27-fold by utilising ball milling cycles of reactive aging, when compared with typical solution-based reactions. This methodology leads to up to a 2600-fold decrease in the solvent required when compared with other leading degradation reactions in the field and a 30-fold decrease in comparison to reported industrial scale PET hydrolysis reactions.

SUBMITTER: Ambrose-Dempster E

PROVIDER: S-EPMC10050947 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Mechanoenzymatic reactions for the hydrolysis of PET.

Ambrose-Dempster Esther E Leipold Leona L Dobrijevic Dragana D Bawn Maria M Carter Eve M EM Stojanovski Gorjan G Sheppard Tom D TD Jeffries Jack W E JWE Ward John M JM Hailes Helen C HC

RSC advances 20230329 15

Recent advances in the enzymatic degradation of poly(ethylene terphthalate) (PET) have led to a number of PET hydrolytic enzymes and mutants being developed. With the amount of PET building up in the natural world, there is a pressing need to develop scalable methods of breaking down the polymer into its monomers for recycling or other uses. Mechanoenzymatic reactions have gained traction recently as a green and efficient alternative to traditional biocatalytic reactions. For the first time we r ...[more]

PMID: 37006375

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