Ontology highlight
ABSTRACT:
SUBMITTER: Conway JRW
PROVIDER: S-EPMC10132756 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
Conway James R W JRW Warren Sean C SC Lee Young-Kyung YK McCulloch Andrew T AT Magenau Astrid A Lee Victoria V Metcalf Xanthe L XL Stoehr Janett J Haigh Katharina K Abdulkhalek Lea L Guaman Cristian S CS Reed Daniel A DA Murphy Kendelle J KJ Pereira Brooke A BA Mélénec Pauline P Chambers Cecilia C Latham Sharissa L SL Lenthall Helen H Deenick Elissa K EK Ma Yuanqing Y Phan Tri T Lim Elgene E Joshua Anthony M AM Walters Stacey S Grey Shane T ST Shi Yan-Chuan YC Zhang Lei L Herzog Herbert H Croucher David R DR Philp Andy A Scheele Colinda L G J CLGJ Herrmann David D Sansom Owen J OJ Morton Jennifer P JP Papa Antonella A Haigh Jody J JJ Nobis Max M Timpson Paul P
Science advances 20230426 17
Aberrant AKT activation occurs in a number of cancers, metabolic syndrome, and immune disorders, making it an important target for the treatment of many diseases. To monitor spatial and temporal AKT activity in a live setting, we generated an Akt-FRET biosensor mouse that allows longitudinal assessment of AKT activity using intravital imaging in conjunction with image stabilization and optical window technology. We demonstrate the sensitivity of the Akt-FRET biosensor mouse using various cancer ...[more]