Ontology highlight
ABSTRACT:
SUBMITTER: Lin D
PROVIDER: S-EPMC10192119 | biostudies-literature | 2023 May
REPOSITORIES: biostudies-literature
Lin Dingchang D Li Xiuyuan X Moult Eric E Park Pojeong P Tang Benjamin B Shen Hao H Grimm Jonathan B JB Falco Natalie N Jia Bill Z BZ Baker David D Lavis Luke D LD Cohen Adam E AE
Nature biotechnology 20230102 5
Recording transcriptional histories of a cell would enable deeper understanding of cellular developmental trajectories and responses to external perturbations. Here we describe an engineered protein fiber that incorporates diverse fluorescent marks during its growth to store a ticker tape-like history. An embedded HaloTag reporter incorporates user-supplied dyes, leading to colored stripes that map the growth of each individual fiber to wall clock time. A co-expressed eGFP tag driven by a promot ...[more]