A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors.
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ABSTRACT:
SUBMITTER: Bateman JR
PROVIDER: S-EPMC2581973 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
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A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors.
Bateman Jack R JR Wu C-ting CT
Genetics 20080914 3
Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the PhiC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis. ...[more]
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