Dataset Information

Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation.

ABSTRACT: Undifferentiated neural stem and progenitor cells (NSPCs) encounter extracellular signals that bind plasma membrane proteins and influence differentiation. Membrane proteins are regulated by N-linked glycosylation, making it possible that glycosylation plays a critical role in cell differentiation. We assessed enzymes that control N-glycosylation in NSPCs and found that loss of the enzyme responsible for generating β1,6-branched N-glycans, N-acetylglucosaminyltransferase V (MGAT5), led to specific changes in NSPC differentiation in vitro and in vivo. Mgat5 homozygous null NSPCs in culture formed more neurons and fewer astrocytes compared with wild-type controls. In the brain cerebral cortex, loss of MGAT5 caused accelerated neuronal differentiation. Rapid neuronal differentiation led to depletion of cells in the NSPC niche, resulting in a shift in cortical neuron layers in Mgat5 null mice. Glycosylation enzyme MGAT5 plays a critical and previously unrecognized role in cell differentiation and early brain development.

SUBMITTER: Yale AR

PROVIDER: S-EPMC10277816 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation.

Yale Andrew R AR Kim Estelle E Gutierrez Brenda B Hanamoto J Nicole JN Lav Nicole S NS Nourse Jamison L JL Salvatus Marc M Hunt Robert F RF Monuki Edwin S ES Flanagan Lisa A LA

Stem cell reports 20230511 6

Undifferentiated neural stem and progenitor cells (NSPCs) encounter extracellular signals that bind plasma membrane proteins and influence differentiation. Membrane proteins are regulated by N-linked glycosylation, making it possible that glycosylation plays a critical role in cell differentiation. We assessed enzymes that control N-glycosylation in NSPCs and found that loss of the enzyme responsible for generating β1,6-branched N-glycans, N-acetylglucosaminyltransferase V (MGAT5), led to specif ...[more]

PMID: 37172586

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Dataset Information

Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation.

Publications

Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation.

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