Ontology highlight
ABSTRACT:
SUBMITTER: Englert D
PROVIDER: S-EPMC10313827 | biostudies-literature | 2023 Jun
REPOSITORIES: biostudies-literature
Englert Daniel D Burger Eva-Maria EM Grün Franziska F Verma Mrigank S MS Lackner Jens J Lampe Marko M Bühler Bastian B Schokolowski Janin J Nienhaus G Ulrich GU Jäschke Andres A Sunbul Murat M
Nature communications 20230630 1
Live-cell RNA imaging with high spatial and temporal resolution remains a major challenge. Here we report the development of RhoBAST:SpyRho, a fluorescent light-up aptamer (FLAP) system ideally suited for visualizing RNAs in live or fixed cells with various advanced fluorescence microscopy modalities. Overcoming problems associated with low cell permeability, brightness, fluorogenicity, and signal-to-background ratio of previous fluorophores, we design a novel probe, SpyRho (Spirocyclic Rhodamin ...[more]