Ontology highlight
ABSTRACT:
SUBMITTER: Laster DJ
PROVIDER: S-EPMC10415806 | biostudies-literature | 2023 Aug
REPOSITORIES: biostudies-literature
Laster Dominique J DJ Akel Nisreen S NS Hendrixson James A JA James Alicen A Crawford Julie A JA Fu Qiang Q Berryhill Stuart B SB Thostenson Jeff D JD Nookaew Intawat I O'Brien Charles A CA Onal Melda M
iScience 20230720 8
Cre-mediated recombination is frequently used for cell type-specific loss of function (LOF) studies. A major limitation of this system is recombination in unwanted cell types. CRISPR interference (CRISPRi) has been used effectively for global LOF in mice. However, cell type-specific CRISPRi, independent of recombination-based systems, has not been reported. To test the feasibility of cell type-specific CRISPRi, we produced two novel knock-in mouse models that achieve gene suppression when used t ...[more]