Dataset Information

Seroprevalence of herpes simplex virus type 1 and type 2 among the migrant workers in Qatar.

ABSTRACT:

Background

Limited data exists on herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infections in migrant populations. This study investigated HSV-1 and HSV-2 seroprevalences and associations among craft and manual workers (CMWs) in Qatar who constitute 60% of Qatar's population.

Methods

A national population-based cross-sectional seroprevalence survey was conducted on the CMW population, all men, between July 26 and September 9, 2020. 2,612 sera were tested for anti-HSV-1 IgG antibodies using HerpeSelect 1 ELISA IgG kits and for anti-HSV-2 IgG antibodies using HerpeSelect 2 ELISA IgG kits (Focus Diagnostics, USA). Univariable and multivariable logistic regression analyses were conducted to identify associations with HSV-1 and HSV-2 infections.

Results

Serological testing identified 2,171 sera as positive, 403 as negative, and 38 as equivocal for HSV-1 antibodies, and 300 sera as positive, 2,250 as negative, and 62 as equivocal for HSV-2 antibodies. HSV-1 and HSV-2 seroprevalences among CMWs were estimated at 84.2% (95% CI 82.8-85.6%) and 11.4% (95% CI 10.1-12.6%), respectively. HSV-1 infection was associated with nationality, educational attainment, and occupation. HSV-2 infection was associated with age, nationality, and educational attainment.

Conclusions

Over 80% of CMWs are infected with HSV-1 and over 10% are infected with HSV-2. The findings highlight the need for sexual health programs to tackle sexually transmitted infections among the CMW population.

SUBMITTER: Nasrallah GK

PROVIDER: S-EPMC10463979 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Publications

Seroprevalence of herpes simplex virus type 1 and type 2 among the migrant workers in Qatar.

Nasrallah Gheyath K GK Dargham Soha R SR Al-Sadeq Duaa W DW Amanullah Fathima H FH Shurrab Farah M FM Nizamuddin Parveen B PB Chemaitelly Hiam H Ayoub Houssein H HH Abdeen Sami S Abdelkarim Ashraf A Daraan Faisal F Ismail Ahmed A Mostafa Nahid N Sahl Mohamed M Suliman Jinan J Tayar Elias E Kasem Hasan Ali HA Agsalog Meynard J A MJA Akkarathodiyil Bassam K BK Alkhalaf Ayat A AA Alakshar Mohamed Morhaf M H MMMH Al-Qahtani Abdulsalam Ali A H AAAH Al-Shedifat Monther H A MHA Ansari Anas A Ataalla Ahmad Ali AA Chougule Sandeep S Gopinathan Abhilash K K V AKKV Poolakundan Feroz J FJ Ranbhise Sanjay U SU Saefan Saed M A SMA Thaivalappil Mohamed M MM Thoyalil Abubacker S AS Umar Inayath M IM Al Kuwari Einas E Coyle Peter P Jeremijenko Andrew A Kaleeckal Anvar Hassan AH Abdul Rahim Hanan F HF Yassine Hadi M HM Al Thani Asmaa A AA Chaghoury Odette O Al Kuwari Mohamed Ghaith MG Farag Elmoubasher E Bertollini Roberto R Al Romaihi Hamad Eid HE Al Khal Abdullatif A Al-Thani Mohamed H MH Abu-Raddad Laith J LJ

Virology journal 20230822 1

<h4>Background</h4>Limited data exists on herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infections in migrant populations. This study investigated HSV-1 and HSV-2 seroprevalences and associations among craft and manual workers (CMWs) in Qatar who constitute 60% of Qatar's population.<h4>Methods</h4>A national population-based cross-sectional seroprevalence survey was conducted on the CMW population, all men, between July 26 and September 9, 2020. 2,612 sera were tested for anti-HSV-1 Ig ...[more]

PMID: 37608372

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Project description:Herpes simplex virus replicates in the nucleus, where new capsids are assembled. It produces procapsids devoid of nucleic acid but containing the preVP22a scaffold protein. These thermo-unstable particles then mature into A-, B- or C-nuclear icosahedral capsids, depending on their ability to shed the proteolytically processed scaffold and incorporation of the viral genome. To study how these viral capsids differ, we performed proteomics studies of highly enriched HSV-1 A-, B- and C-nuclear capsids, relying in part on a novel and powerful flow virometry approach to purify C-capsids. We found that the viral particles contained the expected capsid components and identified several tegument proteins in the C-capsid fraction (pUL21, pUL36, pUL46, pUL48, pUL49, pUL50, pUL51 and pUS10). Moreover, numerous ribosomal, hnRNPs and other host proteins, absent from the uninfected controls, were detected on the capsids with some of them seemingly specific to C-capsids (glycogen synthase, four different keratin-related proteins, fibronectin 1 and PCBP1). A subsequent proteomics analysis was performed to rule out the presence of protein complexes that may share similar density as the viral capsids but do not otherwise interact with them. Using pUL25 or VP5 mutant viruses incapable of assembling C-nuclear or all nuclear capsids, respectively, we confirmed the bulk of our initial findings. Naturally, it will next be important to address the functional relevance of these proteins.IMPORTANCE Much is known about the biology of herpesviruses. This includes their unique ability to traverse the two nuclear envelopes by sequential budding and fusion steps. For HSV-1, this implies the pUL31/pUL34 and pUL17/pUL25 complexes that may favor C-capsid egress. However, this selection process is not clear, nor are all the differences that distinguish A-, B- and C-capsids. The present study probes what proteins compose these capsids, including host proteins. This should open up new research avenues to clarify the biology of this most interesting family of viruses. It also reiterates the use of flow virometry as an innovative tool to purify viral particles.

Dataset Information

Seroprevalence of herpes simplex virus type 1 and type 2 among the migrant workers in Qatar.

Background

Methods

Results

Conclusions

Publications

Seroprevalence of herpes simplex virus type 1 and type 2 among the migrant workers in Qatar.

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