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Droplet-based single-cell joint profiling of histone modifications and transcriptomes.

ABSTRACT: We previously reported Paired-Tag, a combinatorial indexing-based method that can simultaneously map histone modifications and gene expression at single-cell resolution at scale. However, the lengthy procedure of Paired-Tag has hindered its general adoption in the community. To address this bottleneck, we developed a droplet-based Paired-Tag protocol that is faster and more accessible than the previous method. Using cultured mammalian cells and primary brain tissues, we demonstrate its superior performance at identifying candidate cis-regulatory elements and associating their dynamic chromatin state to target gene expression in each constituent cell type in a complex tissue.

SUBMITTER: Xie Y

PROVIDER: S-EPMC10584685 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature

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Droplet-based single-cell joint profiling of histone modifications and transcriptomes.

Xie Yang Y Zhu Chenxu C Wang Zhaoning Z Tastemel Melodi M Chang Lei L Li Yang Eric YE Ren Bing B

Nature structural & molecular biology 20230810 10

We previously reported Paired-Tag, a combinatorial indexing-based method that can simultaneously map histone modifications and gene expression at single-cell resolution at scale. However, the lengthy procedure of Paired-Tag has hindered its general adoption in the community. To address this bottleneck, we developed a droplet-based Paired-Tag protocol that is faster and more accessible than the previous method. Using cultured mammalian cells and primary brain tissues, we demonstrate its superior ...[more]

PMID: 37563440

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