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HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2.


ABSTRACT: In large vertebrate spindles, the majority of microtubules are formed via branching microtubule nucleation, whereby microtubules nucleate along the side of pre-existing microtubules. Hepatoma up-regulated protein (HURP) is a microtubule-associated protein that has been implicated in spindle assembly, but its mode of action is yet to be defined. In this study, we show that HURP is necessary for RanGTP-induced branching microtubule nucleation in Xenopus egg extract. Specifically, HURP stabilizes the microtubule lattice to promote microtubule formation from γ-TuRC. This function is shifted to promote branching microtubule nucleation in the presence of TPX2, another branching-promoting factor, as HURP's localization to microtubules is enhanced by TPX2 condensation. Lastly, we provide a structure of HURP on the microtubule lattice, revealing how HURP binding stabilizes the microtubule lattice. We propose a model in which HURP stabilizes microtubules during their formation, and TPX2 preferentially enriches HURP to microtubules to promote branching microtubule nucleation and thus spindle assembly.

SUBMITTER: Valdez V 

PROVIDER: S-EPMC10769297 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2.

Valdez Venecia V   Ma Meisheng M   Gouveia Bernardo B   Zhang Rui R   Petry Sabine S  

bioRxiv : the preprint server for biology 20231218


In large vertebrate spindles, the majority of microtubules are formed via branching microtubule nucleation, whereby microtubules nucleate along the side of pre-existing microtubules. Hepatoma up-regulated protein (HURP) is a microtubule-associated protein that has been implicated in spindle assembly, but its mode of action is yet to be defined. In this study, we show that HURP is necessary for RanGTP-induced branching microtubule nucleation in <i>Xenopus</i> egg extract. Specifically, HURP stabi  ...[more]

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