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Multiplex PCR for typing strains of Toxoplasma gondii.


ABSTRACT: A multiplex PCR assay was designed for multilocus strain typing of Toxoplasma gondii based on length polymorphism of five microsatellite markers. Eight T. gondii strains already sequenced at these five markers were used as control isolates. This method is simple, rapid, reproducible, and adapted to a large set of isolates.

SUBMITTER: Ajzenberg D 

PROVIDER: S-EPMC1081364 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature

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Multiplex PCR for typing strains of Toxoplasma gondii.

Ajzenberg Daniel D   Dumètre Aurélien A   Dardé Marie-Laure ML  

Journal of clinical microbiology 20050401 4


A multiplex PCR assay was designed for multilocus strain typing of Toxoplasma gondii based on length polymorphism of five microsatellite markers. Eight T. gondii strains already sequenced at these five markers were used as control isolates. This method is simple, rapid, reproducible, and adapted to a large set of isolates. ...[more]

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