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Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains.

ABSTRACT: An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.

SUBMITTER: Lopez-Goni I 

PROVIDER: S-EPMC2566117 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains.

López-Goñi I I   García-Yoldi D D   Marín C M CM   de Miguel M J MJ   Muñoz P M PM   Blasco J M JM   Jacques I I   Grayon M M   Cloeckaert A A   Ferreira A C AC   Cardoso R R   Corrêa de Sá M I MI   Walravens K K   Albert D D   Garin-Bastuji B B  

Journal of clinical microbiology 20080820 10

An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains. ...[more]

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