Unknown

Dataset Information

0

The archaeal TFIIEalpha homologue facilitates transcription initiation by enhancing TATA-box recognition.


ABSTRACT: Transcription from many archaeal promoters can be reconstituted in vitro using recombinant TATA-box binding protein (TBP) and transcription factor B (TFB)--homologues of eukaryal TBP and TFIIB--together with purified RNA polymerase (RNAP). However, all archaeal genomes sequenced to date reveal the presence of TFE, a homologue of the alpha-subunit of the eukaryal general transcription factor, TFIIE. We show that, while TFE is not absolutely required for transcription in the reconstituted in vitro system, it nonetheless plays a stimulatory role on some promoters and under certain conditions. Mutagenesis of the TATA box or reduction of TBP concentration in transcription reactions sensitizes a promoter to TFE addition. Conversely, saturating reactions with TBP de-sensitizes promoters to TFE. These results suggest that TFE facilitates or stabilizes interactions between TBP and the TATA box.

SUBMITTER: Bell SD 

PROVIDER: S-EPMC1083817 | biostudies-literature | 2001 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

The archaeal TFIIEalpha homologue facilitates transcription initiation by enhancing TATA-box recognition.

Bell S D SD   Brinkman A B AB   van der Oost J J   Jackson S P SP  

EMBO reports 20010201 2


Transcription from many archaeal promoters can be reconstituted in vitro using recombinant TATA-box binding protein (TBP) and transcription factor B (TFB)--homologues of eukaryal TBP and TFIIB--together with purified RNA polymerase (RNAP). However, all archaeal genomes sequenced to date reveal the presence of TFE, a homologue of the alpha-subunit of the eukaryal general transcription factor, TFIIE. We show that, while TFE is not absolutely required for transcription in the reconstituted in vitro  ...[more]

Similar Datasets

| S-EPMC20997 | biostudies-literature
| S-EPMC317201 | biostudies-literature
| S-EPMC298155 | biostudies-other
| S-EPMC154304 | biostudies-literature
| S-EPMC3411688 | biostudies-literature
| S-EPMC2150749 | biostudies-literature
| S-EPMC4238354 | biostudies-literature
| S-EPMC2396422 | biostudies-literature
| S-EPMC1135850 | biostudies-other
| S-EPMC4176967 | biostudies-literature