Ontology highlight
ABSTRACT:
SUBMITTER: Bell SD
PROVIDER: S-EPMC1083817 | biostudies-literature | 2001 Feb
REPOSITORIES: biostudies-literature
Bell S D SD Brinkman A B AB van der Oost J J Jackson S P SP
EMBO reports 20010201 2
Transcription from many archaeal promoters can be reconstituted in vitro using recombinant TATA-box binding protein (TBP) and transcription factor B (TFB)--homologues of eukaryal TBP and TFIIB--together with purified RNA polymerase (RNAP). However, all archaeal genomes sequenced to date reveal the presence of TFE, a homologue of the alpha-subunit of the eukaryal general transcription factor, TFIIE. We show that, while TFE is not absolutely required for transcription in the reconstituted in vitro ...[more]