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Activation of archaeal transcription by recruitment of the TATA-binding protein.

ABSTRACT: The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators, Ptr1 and Ptr2, that are members of the Lrp/AsnC family of bacterial transcription regulators. In contrast, this archaeon's RNA polymerase and core transcription factors are of eukaryotic type. Using the M. jannaschii high-temperature in vitro transcription system, we show that Ptr2 is a potent transcriptional activator, and that it conveys its stimulatory effects on its cognate eukaryal-type transcription machinery from an upstream activating region composed of two Ptr2-binding sites. Transcriptional activation is generated, at least in part, by Ptr2-mediated recruitment of the TATA-binding protein to the promoter.

SUBMITTER: Ouhammouch M

PROVIDER: S-EPMC154304 | biostudies-literature | 2003 Apr

REPOSITORIES: biostudies-literature

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Activation of archaeal transcription by recruitment of the TATA-binding protein.

Ouhammouch Mohamed M Dewhurst Robert E RE Hausner Winfried W Thomm Michael M Geiduschek E Peter EP

Proceedings of the National Academy of Sciences of the United States of America 20030411 9

The hyperthermophilic archaeon Methanococcus jannaschii encodes two putative transcription regulators, Ptr1 and Ptr2, that are members of the Lrp/AsnC family of bacterial transcription regulators. In contrast, this archaeon's RNA polymerase and core transcription factors are of eukaryotic type. Using the M. jannaschii high-temperature in vitro transcription system, we show that Ptr2 is a potent transcriptional activator, and that it conveys its stimulatory effects on its cognate eukaryal-type tr ...[more]

PMID: 12692306

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