Improving electrochemical hybridization assays with restriction enzymes.
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ABSTRACT:
SUBMITTER: Zhou X
PROVIDER: S-EPMC10863419 | biostudies-literature | 2024 Feb
REPOSITORIES: biostudies-literature
Publications
Improving electrochemical hybridization assays with restriction enzymes.
Zhou Xingcheng X Zamani Marjon M Austin Katherine K De Bock Marieke M Chaj Ullola Joshua J Riki Smah S Furst Ariel L AL
Chemical communications (Cambridge, England) 20240213 14
Nucleic acids in blood are early indicators of disease that could be detected by point-of-care biosensors if sufficiently sensitive and facile sensors existed. Electrochemical hybridization assays are sensitive and specific but are limited to very short nucleic acids. We have developed a restriction enzyme-assisted electrochemical hybridization (REH) assay for improved nucleic acid detection. By incorporating target-specific restriction enzymes, we detect long nucleic acids, with performance dep ...[more]
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