Genetic analysis of peptidoglycan biosynthesis in mycobacteria: characterization of a ddlA mutant of Mycobacterium smegmatis.
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ABSTRACT:
SUBMITTER: Belanger AE
PROVIDER: S-EPMC111435 | biostudies-literature | 2000 Dec
REPOSITORIES: biostudies-literature
Publications
Genetic analysis of peptidoglycan biosynthesis in mycobacteria: characterization of a ddlA mutant of Mycobacterium smegmatis.
Belanger A E AE Porter J C JC Hatfull G F GF
Journal of bacteriology 20001201 23
A temperature-sensitive mutant of Mycobacterium smegmatis was characterized that contains a mutation in ddlA, the gene encoding D-alanine:D-alanine ligase. Enzymatic assays using recombinant proteins and D-cycloserine susceptibility indicate that the A365V mutation in the SMEG35 DdlA protein causes a reduction in enzymatic activity in vitro and in vivo. ...[more]
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