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Direct Identification of Intact Proteins Using a Low-Resolution Mass Spectrometer with CIDn/ETnoD.

ABSTRACT: Over the past decades, proteomics has become increasingly important and a heavily discussed topic. The identification of intact proteins remains a major focus in this field. While most intact proteins are analyzed using high-resolution mass spectrometry, identifying them through low-resolution mass spectrometry continues to pose challenges. In our study, we investigated the capability of identifying various intact proteins using collision-induced dissociation (CID) and electron transfer without dissociation (ETnoD). Using myoglobin as our test protein, stable product ions were generated with CID, and the identities of the product ions were identified with ETnoD. ETnoD uses a short activation time (AcT, 5 ms) to create sequential charge-reduced precursor ion (CRI). The charges of the fragments and their sequences were determined with corresponding CRI. The product ions can be selected for subsequent CID (termed CIDn) combined with ETnoD for further sequence identification and validation. We refer to this method as CIDn/ETnoD. The use of a multistage CID activation (CIDn) and ETnoD protocol has been applied to several intact proteins to obtain multiple sequence identifications.

SUBMITTER: Kuo CY 

PROVIDER: S-EPMC11228978 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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Direct Identification of Intact Proteins Using a Low-Resolution Mass Spectrometer with CID<sup>n</sup>/ETnoD.

Kuo Cheng-Yu CY   Zheng Yi-Feng YF   Wang Wei-Chen WC   Toh Jie-Teng JT   Hsu Yu-Ming YM   Chien Han-Ju HJ   Chang Chih-Jui CJ   Lai Chien-Chen CC  

Journal of the American Society for Mass Spectrometry 20240621 7

Over the past decades, proteomics has become increasingly important and a heavily discussed topic. The identification of intact proteins remains a major focus in this field. While most intact proteins are analyzed using high-resolution mass spectrometry, identifying them through low-resolution mass spectrometry continues to pose challenges. In our study, we investigated the capability of identifying various intact proteins using collision-induced dissociation (CID) and electron transfer without  ...[more]

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