Ontology highlight
ABSTRACT:
SUBMITTER: Cleland TP
PROVIDER: S-EPMC5549616 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Cleland Timothy P TP DeHart Caroline J CJ Fellers Ryan T RT VanNispen Alexandra J AJ Greer Joseph B JB LeDuc Richard D RD Parker W Ryan WR Thomas Paul M PM Kelleher Neil L NL Brodbelt Jennifer S JS
Journal of proteome research 20170419 5
The analysis of intact proteins (top-down strategy) by mass spectrometry has great potential to elucidate proteoform variation, including patterns of post-translational modifications (PTMs), which may not be discernible by analysis of peptides alone (bottom-up approach). To maximize sequence coverage and localization of PTMs, various fragmentation modes have been developed to produce fragment ions from deep within intact proteins. Ultraviolet photodissociation (UVPD) has recently been shown to p ...[more]