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Ultrasensitive optical detection of strontium ions by specific nanosensor with ultrahigh binding affinity.

ABSTRACT: The release and escape of radioactive materials has posed tremendous threats to the global environment. Among various radioactive elements, ⁹⁰Sr has attracted growing attention due to its long half-life and its tendency to accumulate in bone tissue. Nonetheless, the concentration of ⁹⁰Sr in radioactive waste is exceedingly low, far below the detection limits of currently available strontium-targeting chemical sensors. Herein, we propose an optical nanosensor (Sr²⁺-nanosensor) that exhibits an ultra-low detection limit of 0.5 nM, surpassing the ⁹⁰Sr in the treated radioactive water from the Fukushima. The sensor offers wide sensing range of eight orders of magnitude, rapid response of less than 10 s, and high selectivity against 31 common ions. These excellent performances are attributed to a specific ligand (Sr²⁺-ligand) for Sr²⁺ recognition. The Sr²⁺ is found to be bound by six oxygen atoms from the Sr²⁺-ligand with a stability constant at least two orders higher than that of other traditional ligands. This study offers invaluable insights for the design of Sr²⁺-sensing methodologies as well as a technique for detecting trace amounts of environmental radioactive pollution.

SUBMITTER: Du X

PROVIDER: S-EPMC11297212 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Ultrasensitive optical detection of strontium ions by specific nanosensor with ultrahigh binding affinity.

Du Xinfeng X Xie Hua H Qin Tianyi T Yuan Yihui Y Wang Ning N

Nature communications 20240802 1

The release and escape of radioactive materials has posed tremendous threats to the global environment. Among various radioactive elements, 90Sr has attracted growing attention due to its long half-life and its tendency to accumulate in bone tissue. Nonetheless, the concentration of 90Sr in radioactive waste is exceedingly low, far below the detection limits of currently available strontium-targeting chemical sensors. Herein, we propose an optical nanosensor (Sr2+</sup ...[more]

PMID: 39095434

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Project description:Base excision repair (BER) is an important DNA repair pathway involved in the maintenance of genome stability. As the initiator of BER, DNA glycosylase can remove a damaged base from DNA through cleaving the N-glycosidic bond between the sugar moiety and the damaged base. Accurate quantification of DNA glycosylase is essential for the early diagnosis of various human diseases. However, conventional methods for DNA glycosylase assay usually suffer from poor sensitivity and complex probe design. Herein, we develop a single quantum dot-based nanosensor with multilayer of multiple acceptors for ultrasensitive detection of human alkyladenine DNA glycosylase (hAAG) using apurinic/apyrimidinic endonuclease 1 (APE1)-assisted cyclic cleavage-mediated signal amplification in combination with the DNA polymerase-assisted multiple cyanine 5 (Cy5)-mediated fluorescence resonance energy transfer (FRET). The presence of hAAG induces the cleavage of the hairpin substrate, generating a trigger. The resultant trigger can hybridize with a probe modified with an AP site, initiating the APE1-mediated cyclic cleavage to produce a large number of primers. The primers can subsequently initiate the polymerase-mediated signal amplification with a biotin-modified capture probe as the template, generating the biotin-/multiple Cy5-labeled double-stranded DNAs (dsDNAs). The resultant dsDNAs can assemble onto the QD surface to form the QD-dsDNA-Cy5 nanostructure, leading to efficient FRET from the QD to Cy5 under the excitation of 405 nm. In contrast to the typical QD-based FRET approaches, the assembly of multilayer of multiple Cy5 molecules onto a single QD significantly amplifies the FRET signal. We further verify the FRET model with one donor and multilayered acceptors theoretically and experimentally. This single QD-based nanosensor can sensitively detect hAAG with a detection limit of as low as 4.42 × 10-12 U ?L-1. Moreover, it can detect hAAG even in a single cancer cell, and distinguish the cancer cells from the normal cells. Importantly, this single QD-based nanosensor can be used for the kinetic study and inhibition assay, and it may become a universal platform for the detection of other DNA repair enzymes by designing appropriate DNA substrates.

Dataset Information

Ultrasensitive optical detection of strontium ions by specific nanosensor with ultrahigh binding affinity.

Publications

Ultrasensitive optical detection of strontium ions by specific nanosensor with ultrahigh binding affinity.

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