Ontology highlight
ABSTRACT:
SUBMITTER: Kenyon TK
PROVIDER: S-EPMC115131 | biostudies-literature | 2001 Sep
REPOSITORIES: biostudies-literature
Kenyon T K TK Lynch J J Hay J J Ruyechan W W Grose C C
Journal of virology 20010901 18
Varicella-zoster virus (VZV) codes for a protein serine kinase called ORF47; the herpes simplex virus (HSV) homolog is UL13. No recombinant alphaherpesvirus serine kinase has been biologically active in vitro. We discovered that preservation of the intrinsic kinase activity of recombinant VZV ORF47 required unusually stringent in vitro conditions, including physiological concentrations of polyamines. In this assay, ORF47 phosphorylated two VZV regulatory proteins: the ORF62 protein (homolog of H ...[more]