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Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis.

ABSTRACT: A novel PCR-based restriction fragment length polymorphism analysis of the Brachyspira nox gene was developed. The restriction patterns for Brachyspira hyodysenteriae, B. pilosicoli, B. intermedia, B. murdochii, and B. innocens were highly distinct with two restriction endonucleases only. The assay proved to be user-friendly and robust.

SUBMITTER: Rohde J

PROVIDER: S-EPMC120595 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature

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Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis.

Rohde Judith J Rothkamp Anja A Gerlach Gerald F GF

Journal of clinical microbiology 20020701 7

A novel PCR-based restriction fragment length polymorphism analysis of the Brachyspira nox gene was developed. The restriction patterns for Brachyspira hyodysenteriae, B. pilosicoli, B. intermedia, B. murdochii, and B. innocens were highly distinct with two restriction endonucleases only. The assay proved to be user-friendly and robust. ...[more]

PMID: 12089283

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