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Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity.


ABSTRACT: Cytokines regulate lymphocyte development and differentiation, but precisely how they control these processes is still poorly understood. By using microarray technology to detect cytokine-induced genes, we identified a cDNA encoding Cybr, which was increased markedly in cells incubated with IL-2 and IL-12. The mRNA was most abundant in hematopoietic cells and tissues. The predicted amino acid sequence is similar to that of GRP-1-associated protein (GRASP), a recently identified retinoic acid-induced cytohesin-binding protein. Physical interaction, dependent on the coiled-coil domains of Cybr and cytohesin-1, was demonstrated by coimmunoprecipitation of the overexpressed proteins from 293T cells. Cytohesin-1, in addition to its role in cell adhesion, is a guanine nucleotide-exchange protein activator of ARF GTPases. Acceleration of guanosine 5prime prime or minute-O-(thiotriphosphate) binding to ARF by cytohesin-1 in vitro was enhanced by Cybr. Because the binding protein modified activation of ADP ribosylation factor by cytohesin-1, we designate this cytokine-inducible protein Cybr (cytohesin binder and regulator).

SUBMITTER: Tang P 

PROVIDER: S-EPMC122398 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature

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Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity.

Tang Pingtao P   Cheng Tammy P TP   Agnello Davide D   Wu Chang-You CY   Hissong Bruce D BD   Watford Wendy T WT   Ahn Hyun-Jong HJ   Galon Jerome J   Moss Joel J   Vaughan Martha M   O'Shea John J JJ   Gadina Massimo M  

Proceedings of the National Academy of Sciences of the United States of America 20020226 5


Cytokines regulate lymphocyte development and differentiation, but precisely how they control these processes is still poorly understood. By using microarray technology to detect cytokine-induced genes, we identified a cDNA encoding Cybr, which was increased markedly in cells incubated with IL-2 and IL-12. The mRNA was most abundant in hematopoietic cells and tissues. The predicted amino acid sequence is similar to that of GRP-1-associated protein (GRASP), a recently identified retinoic acid-ind  ...[more]

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