Ontology highlight
ABSTRACT:
SUBMITTER: Ou ZY
PROVIDER: S-EPMC1287784 | biostudies-literature | 2005 Nov
REPOSITORIES: biostudies-literature
Ou Zhi-Ying ZY Liu Na N Chen Chang-Jie CJ Cheng Gang G He Yun-Shao YS
Journal of clinical microbiology 20051101 11
By using the "flow-through hybridization" principle, we developed a new, rapid and accurate reverse dot blot (RDB) method to detect lamivudine resistance-associated YMDD motif variants in hepatitis B virus (HBV) genome. The improved RDB method was very fast at simultaneously detecting HBV YMDD wild-type and mutant motifs. In a blind analysis, 100 samples previously genotyped by DNA clonal sequencing analysis were used to evaluate the sensitivity and specificity of this assay. Conventional restri ...[more]