Unknown

Dataset Information

0

Second harmonic imaging of exocytosis at fertilization.

ABSTRACT: Second harmonic generation offers an important alternative and complement to fluorescence for the imaging of cellular structure and function. Staining the eggs of the sea urchin, Lytechinus pictus, with the styryl dye di-8-ANEPPS, we have observed large changes in both second harmonic generation and two-photon fluorescence after fertilization, consistent with the dynamics of exocytosis of cortical granules. With nonlinear imaging on a scanning microscope, we are able to visualize the wave of exocytosis in real time.

SUBMITTER: Millard AC 

PROVIDER: S-EPMC1305673 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Second harmonic imaging of exocytosis at fertilization.

Millard Andrew C AC   Terasaki Mark M   Loew Leslie M LM  

Biophysical journal 20050422 6

Second harmonic generation offers an important alternative and complement to fluorescence for the imaging of cellular structure and function. Staining the eggs of the sea urchin, Lytechinus pictus, with the styryl dye di-8-ANEPPS, we have observed large changes in both second harmonic generation and two-photon fluorescence after fertilization, consistent with the dynamics of exocytosis of cortical granules. With nonlinear imaging on a scanning microscope, we are able to visualize the wave of exo  ...[more]

Similar Datasets

Unknown Second harmonic generating (SHG) nanoprobes for in vivo imaging.
| S-EPMC2930484 | biostudies-literature
Unknown High-Resolution Plasma Membrane-Selective Imaging by Second Harmonic Generation.
| S-EPMC6249386 | biostudies-literature
Unknown 3D second harmonic generation imaging tomography by multi-view excitation.
| S-EPMC5847324 | biostudies-literature
Unknown Blood vessel imaging using radiofrequency-induced second harmonic acoustic response.
| S-EPMC6195590 | biostudies-literature
Unknown Molecular understanding of label-free second harmonic imaging of microtubules.
| S-EPMC6684603 | biostudies-literature
Unknown Submillisecond second harmonic holographic imaging of biological specimens in three dimensions.
| S-EPMC3831952 | biostudies-literature
Unknown Second Harmonic Generation Imaging Analysis of Collagen Arrangement in Human Cornea.
| S-EPMC4553931 | biostudies-literature
Unknown Noninvasive corneal stromal collagen imaging using two-photon-generated second-harmonic signals.
| S-EPMC1855208 | biostudies-literature
Unknown Second harmonic generation imaging of corneal stroma after infection by Pseudomonas aeruginosa.
| S-EPMC5387403 | biostudies-literature
Unknown Imaging analysis reveals mechanistic differences between first- and second-phase insulin exocytosis.
| S-EPMC2064214 | biostudies-literature