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Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region.

ABSTRACT: Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.

SUBMITTER: Casas I

PROVIDER: S-EPMC1317187 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature

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Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region.

Casas I I Avellon A A Mosquera M M Jabado O O Echevarria J E JE Campos R H RH Rewers M M Perez-Breña P P Lipkin W I WI Palacios G G

Journal of clinical microbiology 20051201 12

Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing. ...[more]

PMID: 16333124

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