Unknown

Dataset Information

0

Crystal structure of a 12 ANK repeat stack from human ankyrinR.


ABSTRACT: Ankyrins are multifunctional adaptors that link specific proteins to the membrane-associated, spectrin- actin cytoskeleton. The N-terminal, 'membrane-binding' domain of ankyrins contains 24 ANK repeats and mediates most binding activities. Repeats 13-24 are especially active, with known sites of interaction for the Na/K ATPase, Cl/HCO(3) anion exchanger, voltage-gated sodium channel, clathrin heavy chain and L1 family cell adhesion molecules. Here we report the crystal structure of a human ankyrinR construct containing ANK repeats 13-24 and a portion of the spectrin-binding domain. The ANK repeats are observed to form a contiguous spiral stack with which the spectrin-binding domain fragment associates as an extended strand. The structural information has been used to construct models of all 24 repeats of the membrane-binding domain as well as the interactions of the repeats with the Cl/HCO(3) anion exchanger and clathrin. These models, together with available binding studies, suggest that ion transporters such as the anion exchanger associate in a large central cavity formed by the ANK repeat spiral, while clathrin and cell adhesion molecules associate with specific regions outside this cavity.

SUBMITTER: Michaely P 

PROVIDER: S-EPMC136955 | biostudies-literature | 2002 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Crystal structure of a 12 ANK repeat stack from human ankyrinR.

Michaely Peter P   Tomchick Diana R DR   Machius Mischa M   Anderson Richard G W RG  

The EMBO journal 20021201 23


Ankyrins are multifunctional adaptors that link specific proteins to the membrane-associated, spectrin- actin cytoskeleton. The N-terminal, 'membrane-binding' domain of ankyrins contains 24 ANK repeats and mediates most binding activities. Repeats 13-24 are especially active, with known sites of interaction for the Na/K ATPase, Cl/HCO(3) anion exchanger, voltage-gated sodium channel, clathrin heavy chain and L1 family cell adhesion molecules. Here we report the crystal structure of a human ankyr  ...[more]

Similar Datasets

| S-EPMC2242602 | biostudies-literature
| S-EPMC3107870 | biostudies-literature
| S-EPMC1952456 | biostudies-literature
| S-EPMC4461331 | biostudies-literature
| S-EPMC6211530 | biostudies-literature
| S-EPMC4822990 | biostudies-literature
| S-EPMC11316315 | biostudies-literature
| S-EPMC2475683 | biostudies-literature
| S-EPMC149896 | biostudies-literature
| S-EPMC5754798 | biostudies-literature