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Generation of a catalytic module on a self-folding RNA.

ABSTRACT: It is theoretically possible to obtain a catalytic site of an artificial ribozyme from a random sequence consisting of a limited numbers of nucleotides. However, this strategy has been inadequately explored. Here, we report an in vitro selection technique that exploits modular construction of a structurally constrained RNA to acquire a catalytic site for RNA ligation from a short random sequence. To practice the selection, a sequence of 30 nucleotides was located close to the putative reaction site in a derivative of a naturally occurring self-folding RNA whose crystal structure is known. RNAs whose activity depended on the starting three-dimensional structure were selected with 3'-5' ligation specificity, indicating that the strategy can be used to acquire a variety of catalytic sites and other functional RNA modules.

SUBMITTER: Yoshioka W

PROVIDER: S-EPMC1370678 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature

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Generation of a catalytic module on a self-folding RNA.

Yoshioka Wataru W Ikawa Yoshiya Y Jaeger Luc L Shiraishi Hideaki H Inoue Tan T

RNA (New York, N.Y.) 20041103 12

It is theoretically possible to obtain a catalytic site of an artificial ribozyme from a random sequence consisting of a limited numbers of nucleotides. However, this strategy has been inadequately explored. Here, we report an in vitro selection technique that exploits modular construction of a structurally constrained RNA to acquire a catalytic site for RNA ligation from a short random sequence. To practice the selection, a sequence of 30 nucleotides was located close to the putative reaction s ...[more]

PMID: 15525711

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