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RNAi dependent epigenetic marks on a geminivirus promoter.


ABSTRACT: Nicotiana benthamiana plants were stably transformed with an intron-spliced dsRNA producing construct cognate to bidirectional promoter of African cassava mosaic geminivirus (ACMV) DNA A. Transgenic lines expressed multiple siRNAs species upon ACMV infection. The de novo DNA methylation and an increased proportion of histone H3 Lysine-9 methylation (H3K9) at intergenic region (IGR) of ACMV DNA A were observed.

SUBMITTER: Dogar AM 

PROVIDER: S-EPMC1402257 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature

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RNAi dependent epigenetic marks on a geminivirus promoter.

Dogar Afzal Muhammad AM  

Virology journal 20060130


Nicotiana benthamiana plants were stably transformed with an intron-spliced dsRNA producing construct cognate to bidirectional promoter of African cassava mosaic geminivirus (ACMV) DNA A. Transgenic lines expressed multiple siRNAs species upon ACMV infection. The de novo DNA methylation and an increased proportion of histone H3 Lysine-9 methylation (H3K9) at intergenic region (IGR) of ACMV DNA A were observed. ...[more]

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