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A selective turn-on fluorescent sensor for imaging copper in living cells.

ABSTRACT: We present the synthesis, properties, and biological applications of Coppersensor-1 (CS1), a new water-soluble, turn-on fluorescent sensor for intracellular imaging of copper in living biological samples. CS1 utilizes a BODIPY reporter and thioether-rich receptor to provide high selectivity and sensitivity for Cu+ over other biologically relevant metal ions, including Cu2+, in aqueous solution. This BODIPY-based probe is the first Cu+-responsive sensor with visible excitation and emission profiles and gives a 10-fold turn-on response for detecting this ion. Confocal microscopy experiments further establish that CS1 is membrane-permeable and can successfully monitor intracellular Cu+ levels within living cells.

SUBMITTER: Zeng L

PROVIDER: S-EPMC1414792 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature

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A selective turn-on fluorescent sensor for imaging copper in living cells.

Zeng Li L Miller Evan W EW Pralle Arnd A Isacoff Ehud Y EY Chang Christopher J CJ

Journal of the American Chemical Society 20060101 1

We present the synthesis, properties, and biological applications of Coppersensor-1 (CS1), a new water-soluble, turn-on fluorescent sensor for intracellular imaging of copper in living biological samples. CS1 utilizes a BODIPY reporter and thioether-rich receptor to provide high selectivity and sensitivity for Cu+ over other biologically relevant metal ions, including Cu2+, in aqueous solution. This BODIPY-based probe is the first Cu+-responsive sensor with visible excitation and emission profil ...[more]

PMID: 16390096

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