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A turn-on fluorescent sensor for detecting nickel in living cells.

ABSTRACT: We present the synthesis and properties of Nickelsensor-1 (NS1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively responding to Ni(2+) in aqueous solution and in living cells. NS1 combines a BODIPY chromophore and a mixed N/O/S receptor to provide good selectivity for Ni(2+) over a range of biologically abundant metal ions in aqueous solution. In addition to these characteristics, confocal microscopy experiments further show that NS1 can be delivered into living cells and report changes in intracellular Ni(2+) levels in a respiratory cell model.

SUBMITTER: Dodani SC

PROVIDER: S-EPMC2810492 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature

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A turn-on fluorescent sensor for detecting nickel in living cells.

Dodani Sheel C SC He Qiwen Q Chang Christopher J CJ

Journal of the American Chemical Society 20091201 50

We present the synthesis and properties of Nickelsensor-1 (NS1), a new water-soluble, turn-on fluorescent sensor that is capable of selectively responding to Ni(2+) in aqueous solution and in living cells. NS1 combines a BODIPY chromophore and a mixed N/O/S receptor to provide good selectivity for Ni(2+) over a range of biologically abundant metal ions in aqueous solution. In addition to these characteristics, confocal microscopy experiments further show that NS1 can be delivered into living cel ...[more]

PMID: 19950946

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