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A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging.

ABSTRACT: The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound YZ-A4 as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor YZ-A4 to selenocysteine was from 0 to 32 ?M, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that YZ-A4 could be used for fluorescence imaging of selenocysteine in living cells.

SUBMITTER: Wang Z

PROVIDER: S-EPMC7506812 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging.

Wang Zongcheng Z Zheng Huihuang H Zhang Chengliang C Tang Dongfang D Wu Qiyao Q Dessie Wubliker W Jiang Yuren Y

Sensors (Basel, Switzerland) 20200824 17

The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound <b>YZ-A4</b> as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the senso ...[more]

PMID: 32846934

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