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A selective, cell-permeable optical probe for hydrogen peroxide in living cells.


ABSTRACT: We present the synthesis, properties, and biological applications of Peroxyfluor-1 (PF1), a new type of optical probe for intracellular imaging of hydrogen peroxide in living biological samples. PF1 utilizes a boronate deprotection mechanism to provide unprecedented selectivity and optical dynamic range for detecting H2O2 in aqueous solution over similar reactive oxygen species including superoxide, nitric oxide, tert-butyl hydroperoxide, and hydroxyl radical. We further demonstrate the value of this reagent for biological applications by imaging changes in [H2O2] in living mammalian cells.

SUBMITTER: Chang MC 

PROVIDER: S-EPMC1434465 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature

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A selective, cell-permeable optical probe for hydrogen peroxide in living cells.

Chang Michelle C Y MC   Pralle Arnd A   Isacoff Ehud Y EY   Chang Christopher J CJ  

Journal of the American Chemical Society 20041201 47


We present the synthesis, properties, and biological applications of Peroxyfluor-1 (PF1), a new type of optical probe for intracellular imaging of hydrogen peroxide in living biological samples. PF1 utilizes a boronate deprotection mechanism to provide unprecedented selectivity and optical dynamic range for detecting H2O2 in aqueous solution over similar reactive oxygen species including superoxide, nitric oxide, tert-butyl hydroperoxide, and hydroxyl radical. We further demonstrate the value of  ...[more]

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