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A red-emitting naphthofluorescein-based fluorescent probe for selective detection of hydrogen peroxide in living cells.


ABSTRACT: We report the synthesis, properties, and cellular application of Naphtho-Peroxyfluor-1 (NPF1), a new fluorescent indicator for hydrogen peroxide based on a red-emitting naphthofluorescein platform. Owing to its boronate cages, NPF1 features high selectivity for hydrogen peroxide over a panel of biologically relevant reactive oxygen species (ROS), including superoxide and nitric oxide, as well as excitation and emission profiles in the far-red region of the visible spectrum (>600nm). Flow cytometry experiments in RAW264.7 macrophages establish that NPF1 can report changes in peroxide levels in living cells.

SUBMITTER: Albers AE 

PROVIDER: S-EPMC2590631 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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A red-emitting naphthofluorescein-based fluorescent probe for selective detection of hydrogen peroxide in living cells.

Albers Aaron E AE   Dickinson Bryan C BC   Miller Evan W EW   Chang Christopher J CJ  

Bioorganic & medicinal chemistry letters 20080814 22


We report the synthesis, properties, and cellular application of Naphtho-Peroxyfluor-1 (NPF1), a new fluorescent indicator for hydrogen peroxide based on a red-emitting naphthofluorescein platform. Owing to its boronate cages, NPF1 features high selectivity for hydrogen peroxide over a panel of biologically relevant reactive oxygen species (ROS), including superoxide and nitric oxide, as well as excitation and emission profiles in the far-red region of the visible spectrum (>600nm). Flow cytomet  ...[more]

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