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Spatially-resolved intracellular sensing of hydrogen peroxide in living cells.

ABSTRACT: Understanding intracellular redox chemistry requires new tools for the site-specific visualization of intracellular oxidation. We have developed a spatially-resolved intracellular sensor of hydrogen peroxide, HyPer-Tau, for time-resolved imaging in live cells. This sensor consists of a hydrogen peroxide-sensing protein tethered to microtubules. We demonstrate the use of the HyPer-Tau sensor for three applications; dose-dependent response of human cells to exogenous hydrogen peroxide, a model immune response of mouse macrophages to stimulation by bacterial toxin, and a spatially-resolved response to localized delivery of hydrogen peroxide. These results demonstrate that HyPer-Tau can be used as an effective tool for tracking changes in spatially localized intracellular hydrogen peroxide and for future applications in redox signaling.

SUBMITTER: Warren EA

PROVIDER: S-EPMC4653655 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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Spatially-resolved intracellular sensing of hydrogen peroxide in living cells.

Warren Emilie A K EA Netterfield Tatiana S TS Sarkar Saheli S Kemp Melissa L ML Payne Christine K CK

Scientific reports 20151120

Understanding intracellular redox chemistry requires new tools for the site-specific visualization of intracellular oxidation. We have developed a spatially-resolved intracellular sensor of hydrogen peroxide, HyPer-Tau, for time-resolved imaging in live cells. This sensor consists of a hydrogen peroxide-sensing protein tethered to microtubules. We demonstrate the use of the HyPer-Tau sensor for three applications; dose-dependent response of human cells to exogenous hydrogen peroxide, a model imm ...[more]

PMID: 26585385

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