Ontology highlight
ABSTRACT:
SUBMITTER: Zhu C
PROVIDER: S-EPMC1458854 | biostudies-literature | 2006 Apr
REPOSITORIES: biostudies-literature
Zhu Changjun C Lau Eric E Schwarzenbacher Robert R Bossy-Wetzel Ella E Jiang Wei W
Proceedings of the National Academy of Sciences of the United States of America 20060407 16
We have examined the role of PRC1, a midzone-associated, microtubule bundling, Cdk substrate protein, in regulating the spatiotemporal formation of the midzone in HeLa cells. Cdk-mediated phosphorylation of PRC1 in early mitosis holds PRC1 in an inactive monomeric state. During the metaphase-to-anaphase transition, PRC1 is dephosphorylated, promoting PRC1 oligomerization. Using time-lapse video microscopy, RNA interference, 3D immunofluorescence reconstruction imaging, and rescue experiments, we ...[more]