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Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay.


ABSTRACT: The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information.

SUBMITTER: Francois P 

PROVIDER: S-EPMC1479209 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature

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Rapid Staphylococcus aureus agr type determination by a novel multiplex real-time quantitative PCR assay.

Francois Patrice P   Koessler Thibaud T   Huyghe Antoine A   Harbarth Stephan S   Bento Manuela M   Lew Daniel D   Etienne Jérôme J   Pittet Didier D   Schrenzel Jacques J  

Journal of clinical microbiology 20060501 5


The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information. ...[more]

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