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Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes.

ABSTRACT: We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.

SUBMITTER: Barfod L

PROVIDER: S-EPMC1489712 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature

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Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes.

Barfod Lea L Nielsen Morten A MA Turner Louise L Dahlbäck Madeleine M Jensen Anja T R AT Hviid Lars L Theander Thor G TG Salanti Ali A

Infection and immunity 20060701 7

We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antige ...[more]

PMID: 16790811

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VAR2CSA-Mediated Host Defense Evasion of <i>Plasmodium falciparum</i> Infected Erythrocytes in Placental Malaria.

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