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Close identity of a pressure-stabilized intermediate with a kinetic intermediate in protein folding.

ABSTRACT: Atomic detailed structural study of a transiently existing folding intermediate is severely limited because of its short life. In ubiquitin, we found that a pressure-stabilized equilibrium conformer shares a common structural feature with the proline-trapped kinetic intermediate found in a pulse-labeling (1)H(2)H exchange NMR study [Briggs, M. S. & Roder, H. (1992) Proc. Natl. Acad. Sci. USA 89, 2017-2021]. The conformer is locally unfolded in the entire segment from residues 33 to 42 and in C-terminal residues 70-76. The close structural identity of an equilibrium intermediate stabilized under pressure with a transiently observed folding intermediate is likely to be general in terms of a folding funnel common to both experiments.

SUBMITTER: Kitahara R

PROVIDER: S-EPMC152264 | biostudies-literature | 2003 Mar

REPOSITORIES: biostudies-literature

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Close identity of a pressure-stabilized intermediate with a kinetic intermediate in protein folding.

Kitahara Ryo R Akasaka Kazuyuki K

Proceedings of the National Academy of Sciences of the United States of America 20030310 6

Atomic detailed structural study of a transiently existing folding intermediate is severely limited because of its short life. In ubiquitin, we found that a pressure-stabilized equilibrium conformer shares a common structural feature with the proline-trapped kinetic intermediate found in a pulse-labeling (1)H(2)H exchange NMR study [Briggs, M. S. & Roder, H. (1992) Proc. Natl. Acad. Sci. USA 89, 2017-2021]. The conformer is locally unfolded in the entire segment from residues 33 to 42 and in C-t ...[more]

PMID: 12629216

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